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Abstract

RP-HPLC method for simultaneous estimation of telmisartan and hydrochlorothiazide in tablet dosage form

Author(s): SB Wankhede, MR Tajne, KR Gupta, SG Wadodkar
University Department of Pharmaceutical Sciences, Nagpur University, Nagpur - 440 033, India

Correspondence Address:
S B Wankhede c/o. R. N. Sakpal, Rachana Appartment, Pavnaskar Layout, Tope Nagar, Amravati - 444602 India E-mail: [email protected]


The present work describes a validated reverse phase high performance liquid chromatographic method for simultaneous estimation of telmisartan and hydrochlorothiazide in tablet formulation. Chromatography was performed on a ODS Hypersil C18 (25 cm×4.6 mm I.D) column from thermo in isocratic mode with mobile phase containing acetonitrile:0.05 M KH2PO4 pH 3.0 (60:40). The flow rate was 1.0 ml/min and the eluent was monitored at 271 nm. The selected chromatographic conditions were found to effectively separate telmisartan (RT- 5.19 min) and hydrochlorothiazide (RT- 2.97 min). Linearity for telmisartan and hydrochlorothiazide were found in the range of 4.1-20.48 µg/ml and 1.28-6.4 µg/ml, respectively. The proposed method was found to be accurate, precise, reproducible and specific and can be used for simultaneous analysis of these drugs in tablet formulation.

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