All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Spectrofluorimetric determination of ?-tocopherol in capsules and human plasma

Author(s): Fatma Demirkaya-Miloglu, Y Kadioglu, O Senol, ME Yaman
Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, Erzurum-25240, Turkey

Correspondence Address:
Fatma Demirkaya-Miloglu Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, Erzurum-25240 Turkey E‑mail: [email protected]


A simple, sensitive and rapid spectrofluorimetric method for determination of α-tocopherol in pharmaceutical capsule and human plasma was developed and validated. The native fluorescence of α-tocopherol was measured at 334 nm with excitation at 291 nm, after extraction of α-tocopherol from human plasma hexane:dichloromethane mixture. The calibration curves were linear (R≥0.9993) in the concentration range of 0.25-2.5 μg/ml of α-tocopherol in both standard solutions and plasma samples. The developed method was directly and easily applied for determination of α-tocopherol in the plasma of healthy volunteers and different type of bladder cancer and stomach cancer patients and also pharmaceutical capsule.

Full-Text | PDF