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Abstract

Study of the Correlation between Expression of P-Glycoprotein, Livin, Survivin and Drug Resistance in Human Gastric Cancer Cell Line OCUM-2MD3/L-OHP

Author(s): Jing Zhang, L. Zhang, Y. Zhang and Yan Zhang*
Department of Clinical Laboratory, The First Affiliated Hospital of Xi???an Jiaotong University, Xi???an, Shaanxi Province 710061, 1Department of Medicine, Xi???an Peihua University, Xi???an, Shaanxi Province 710125, 2Department of Spleen and Stomach Diseases, Xi'an Traditional Chinese Medicine Hospital, Xi'an, Shaanxi Province 710021, China

Correspondence Address:
Yan Zhang, Department of Spleen and Stomach Diseases, Xi'an Traditional Chinese Medicine Hospital, Xi'an, Shaanxi Province 710021, China, E-mail: 76899846@qq.com


P-glycoprotein, livin, and survivin are apoptosis inhibitory proteins. This study was to clarify the correlation between expression of P-glycoprotein, livin, survivin, and drug resistance in the human gastric cancer resistant cell line OCUM-2MD3/oxaliplatin. The highly invasive metastatic gastric cancer cell line OCUM- 2MD3 was utilized as experimental material. The oxaliplatin-resistant cell line, OCUM-2MD3/oxaliplatin, was established by inducing OCUM-2MD3 cells with oxaliplatin. Flow cytometry was employed to analyze the cell cycle and apoptosis of OCUM-2MD3/oxaliplatin cells. The sensitivity of OCUM-2MD3/oxaliplatin cells to drugs was determined using cell counting kit-8 assay. Real-time quantitative polymerase chain reaction and Western blot analysis were performed to determine the gene and protein expression levels of P-glycoprotein, livin, and survivin in OCUM-2MD3 and OCUM-2MD3/oxaliplatin cells, establishing the association between expression of these genes and proteins and drug resistance of OCUM-2MD3/oxaliplatin cells. Gene and protein expression levels of P-glycoprotein, livin, and survivin were markedly upregulated in OCUM-2MD3/ oxaliplatin cells relative to OCUM-2MD3 cells. P-glycoprotein and livin expression exhibited a positive correlation with cisplatin resistance in OCUM-2MD3/oxaliplatin cells, whereas survivin expression showed a negative correlation with resistance. The expression levels of P-glycoprotein, livin, and survivin are closely associated with drug resistance in the human gastric cancer resistant cell line OCUM-2MD3/oxaliplatin. A theoretical basis was provided for development of novel therapeutic strategies targeting these molecules and contributes to enhancing clinical efficacy in gastric cancer patients.

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