Study on the Mechanism of Soothing Liver Mixture against Liver Injury induced by Antituberculosis Drugs through Lipopolysaccharide/Toll-Like Receptor 4/Nuclear Factor Kappa B Signal Pathway
Department of Pharmacy, Affiliated Hangzhou Chest Hospital, Zhejiang University School of Medicine, Zhejiang 310053, 1Department of Traditional Chinese Medicine, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang 310012, China
X. Hu, Department of Traditional Chinese Medicine, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang 310012, China, E-mail: email@example.com
Based on plasma endotoxin/toll-like receptor-4/nuclear factor-kappa B signal pathway to investigate the protective effect of soothing liver mixture on mice with liver injury induced by antituberculosis drugs. A total of 72 Sprague Dawley male rats were randomly divided into blank control group, model group, silymarin group and soothing liver mixture group (low, medium and high dosage), with 12 rats in each group. 16 h after the last administration, the pathological changes of liver tissue were observed by hematoxylin eosin staining; blood samples were taken from eyeball to detect liver function indexes (alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase) oxidative stress indexes (superoxide dismutase, malondialdehyde, glutathione peroxidase) and inflammatory factors (interleukin- 1beta, interleukin-6, tumor necrosis factor-alpha) and the content of plasma endotoxin in plasma; the microRNA expression levels of toll-like receptor-4 and nuclear factor-kappa B in rat liver were detected by real time reverse transcription–polymerase chain reaction. Soothing liver mixture can alleviate hepatocyte injury, effectively improve liver function in rats, attenuate oxidative stress and inflammatory reactions in the body, reduce plasma endotoxin content and inhibit hepatic toll-like receptor-4 and nuclear factorkappa B microRNA and protein expression, the higher the drug dose, the better the silymarin was similar to the high dose soothing liver mixture efficacy. Soothing liver mixture has a better protective effect on rats with liver injury induced by antituberculosis drugs; the mechanism may involve the regulation of plasma endotoxin/toll-like receptor-4/nuclear factor-kappa B signaling pathway the alleviation of the body's oxidative stress and inflammatory reaction.