Abstract

People and pharmaceuticals of world are attracting towards herbs and alternates for prophylactic and therapeutic uses based on their folklore utilization. Hygrophila spinosa plant was extensively used in traditional systems of medicine for treating various ailments like jaundice, rheumatism, anemia, inflammation, pain, urinary infections, oedema and gout. Alternative medicine including cow urine distillate (ark) maintains well-being and in recent decades researches on cow urine distillate are gaining interest unveiling its therapeutic potential scientifically. It is claimed as novel pharmaceutical composition particularly for its bio enhancer activities of commonly used antibiotics, anti-fungal and anti-cancer drugs. This study was performed with the aim to evaluate 28 d repeated dose oral safety profile of Hygrophila spinosa extract and Kosli cow urine distillate using hemato-biochemical parameters, antioxidant status and histopathological parameters in Albino rats. The findings of study showed no significant difference in average body weight gain, hemato-biochemical parameters, oxidative status and histopathological examination of control and various treatment group rats. Hygrophila spinosa, cow urine distillate and their combination are safe for treatment of this duration.