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Abstract

Walnut Green Husk Extract Suppresses Ovarian Cancer Cell Proliferation, Migration and Invasion via Modulating microRNA-144-3p

Author(s): Yanan Liu, Lin Yang, Y. Gu, L. Guo and Qi Guo*
Department of Obstetrics and Gynecology, 1Intensive Care Unit, 2Department of Obstetrics and Gynecology Nursing, 3Department of Radiotherapy, Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province 215006, China

Correspondence Address:
Qi Guo, Department of Radiotherapy, Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province 215006, China, E-mail: guoqi456258@163.com


Previous reports have indicated that walnut green husk extract has an inhibitory effect on human cancer development. This project aimed to explore the influence of walnut green husk extract on ovarian cancer. SKOV3 cells were exposed to various doses of walnut green husk extract or transfected with microRNAcontrol or microRNA-144-3p. Meanwhile, anti-microRNA-144-3p or anti-microRNA-control-transfected SKOV3 cells were treated with high-dose walnut green husk extract. Cell counting kit-8, flow cytometry and Transwell monitored cell activity, cycle progression, migration and invasion. Cyclin D1, matrix metallopeptidase 2, matrix metallopeptidase 9, microRNA-144-3p contents were appraised via Western blot or real-time quantitative polymerase chine reaction. After treatment with different concentrations of walnut green husk extract, cell activity, cycle progression, migration, invasion and cyclin D1, matrix metallopeptidase 2 and matrix metallopeptidase 9 protein expression were decreased and microRNA-144-3p level increased in a dose-dependent manner. MicroRNA-144-3p upregulation might hinder cell activity, cycle progression, migration and invasion. Additionally, miR-144-3p knockdown might abolish the repression of high-dose walnut green husk extract on Janus kinase 2 and signal transducer, and activator of transcription 3 expression. Walnut green husk extract might block ovarian cancer development via modulating the microRNA-144-3p/ Janus kinase 2/signal transducer and activator of transcription 3 pathway.

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