- *Corresponding Author:
- Jinnan Gao
Department of Breast Surgery, Shan Xi Bethune Hospital, Taiyuan ShanXi 030032, P. R. China
E-mail: [email protected]
|This article was originally published in a special issue, “Recent Trends in Biomedical Research”|
|Indian J Pharm Sci 2020:82(1)spl issue1;XX-XX|
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms
To investigate the therapeutic effect of anthracycline chemotherapy in advanced breast cancer and its influence on estradiol and tumor size, 136 breast cancer patients in Shan Xi Bethune Hospital were divided into anthracycline chemotherapy group and non anthracycline chemotherapy group. The clinical effects of anthracycline chemotherapy group and non-anthracycline chemotherapy group were observed. The levels of estrone, estradiol and follicle stimulating hormone before and after treatment were measured. The tumor size, adverse reactions and 2-y survival rate of anthracycline chemotherapy group and non anthracycline chemotherapy group were measured after 1-3 courses of treatment. The total effective rate of anthracycline chemotherapy group and non-anthracycline chemotherapy group was 64.7 and 23.5 %, respectively and the total effective rate of non anthracycline chemotherapy group was significantly lower than that of anthracycline chemotherapy group (p<0.05). There was no significant difference in the comparison of serum estrone, estradiol and follicle stimulating hormone between the anthracycline chemotherapy group and non-anthracycline chemotherapy group (p<0.05). Compared to the anthracycline chemotherapy group, the level of serum estrone and estradiol in non-anthracycline chemotherapy group was significantly higher, compared to the non-anthracycline chemotherapy group, the level of serum follicle stimulating hormone in the anthracycline chemotherapy group was significantly higher (p<0.05). There was no significant difference in tumor size between the anthracycline chemotherapy group and non-anthracycline chemotherapy group (p>0.05). After treatment, the tumor volume of each group gradually decreased with increased time of drug exposure and the degree of tumor reduction in the non-anthracycline chemotherapy group was lower than that of the anthracycline chemotherapy group (p<0.05). Adverse reactions in both the groups were, the number of leucopenia cases in the anthracycline chemotherapy group was 14 while that in the non-anthracycline chemotherapy group was 17. The number of adverse reactions in the non-anthracycline chemotherapy group was more than that in the anthracycline chemotherapy group, but the difference was not significant (p>0.05). After 2 y of follow-up, the survival rate of anthracycline chemotherapy group was significantly higher than that of non-anthracycline chemotherapy group (p<0.05). The effect of anthracycline on advanced breast cancer was better, the level of serum estradiol decreased, and the volume of tumor decreased.
Breast cancer, anthracyclines, E2, clinical efficacy
Breast cancer is one of the common tumors in women, and it is also one of the reasons for the high mortality rate of women cancer patients. Recently significant progress has been made in treating breast cancer. Surgical resection is also an current effective treatment mode, which improved the quality of life of patients for 5 to 10 y. However, there is still metastasis and recurrence after radical mastectomy[1,2]. Early diagnosis and comprehensive treatment of breast cancer can effectively improve the 5-y and 10-y survival rate of patients. However, 30 % of breast cancer patients still have the possibility of recurrence and metastasis, which causes many difficulties in clinical treatment. At present, anthracycline drugs such as daunorubicin, doxorubicin and Adriamycin have significant effect in clinical tumor management and are commonly used in clinical medicine. These drugs exert definite effect in the treatment of breast cancer, high mortality, high tolerance and low recurrence. In this paper, epirubicin is selected as anthracycline drug. Epirubicin is one of the derivatives developed on the basis of anthracycline. Compared to its derivatives, epirubicin has a lower incidence of cardiotoxicity and its leakage effect is very good. It enters into cancer cell interior and controls disease progress by inhibiting nucleic acid generation and mitosis of cancer cells[5,6]. The growth and development of human breast tissue will be affected by many endocrine hormones, such as estrone (E1), estradiol (E2) and follicle stimulating hormone (FSH). Estrogens are one of the important endocrine hormones of women, which play an important role in the diagnosis of breast cancer. In this paper, the effect of anthracycline chemotherapy in the treatment of advanced breast cancer and effects on E2 levels and tumor size were studied.
One hundred and thirty six breast cancer patients including 2 males reported at the Shan Xi Bethune Hospital from March 2014 to July 2016 were included in this study. The age of the patients was 21 y at the minimum and 74 y at the maximum. According to the order of admission, the number of patients in the anthracycline chemotherapy (NH) group and the same number were in the non-anthracycline chemotherapy group (conventional drugs treated, CG) with 68 cases in each group. The average age of patients in NH group was 45.23±3.03 y. The average age of patients in CG group was 45.31±2.87 y. There was no difference in the comparative clinical data between the NH group and CG group as shown in Table 1.
Table 1: Clinical Data
|Group||n||Average age (y)||BMI||Cancer measurement||Marital status|
The inclusion criteria were, advanced breast cancer without metastasis, aged between 21-75 y, patients with complete admission and screening data and those in good physical condition during the study period. The exclusion criteria were those with abnormal function of other organs, those with multiple types of tumor at the same time, pregnant or lactating patients and those with poor communication ability.
Patients in the two groups were given nutrition support, dexamethasone and 5-HT3 receptor blocker before chemotherapy to prevent adverse reactions. In the NH group, patients were given epirubicin intravenously at a dose of 75 mg/m2 for one day and docetaxel was given the same dosage as epirubicin for one day, once in the course of treatment; in the CG group, capecitabine was given at a dose of 2 g/m2, no more than 4 g/m2 per day, for 2 w, during the course of treatment. The course of treatment was 21 d in the NH and the CG groups.
The clinical effects were observed in the NH and the CG groups. The levels of E1, E2 and FSH were monitored in both the NH and CG groups. The tumor size was monitored after 1-3 courses of treatment in both groups. Adverse reactions in both groups were monitored and compared. Survival rate in both the groups was monitored through follow-up for 2 y.
E1, E2 and FSH levels were determined by collecting 5 ml of venous blood when the patients in the group were fasting once before and once after treatment. Plasma was separated by centrifugation and the changes in E1, E2 and FSH in plasma were measured using ELISA. Measurement of tumor size was using the select Siemens acusons 2000 ABVS (automated breast volume scanner) system, 14l5 bv high-frequency linear array probe the frequency of which was set to 11 MHz, scans with lateral, central and medial breast records were sent to the ABVS workstation, in which using the 3-D reconstruction method coronal and sagittal plane data was included, The largest section was selected to calculate the volume of the foci. Complete relief was considered when the pain on the affected side disappeared, the nipple had no beneficial fluid and when the lesions were absent. Partial remission was when more than 30 % of lesion was found to shrink. Disease was considered as stable when shrinkage in the diameter and volume of the tumor could be observed, but not to achieve partial remission. Disease was considered to progress when more than 20 % of the lesions increase in volume, or when new lesions appeared.
The changes in E1, E2 and FSH levels, survival rate and tumour volume before and after treatment were statistically analysed using GraphPad Prism 8 software. Chi square test was used for n (%), variable was used for and LSD t (least significant difference) or Bonferroni test was used for comparison between the 2 groups. Repeated measurement analysis of variance and log rank test were used for comparison between the 2 groups at different time points, with p<0.05 as the difference remarkable.
The total effective rate of NH group and CG group was 64.7 and 23.5 %, respectively and the total effective rate of CG group was significantly lower (p<0.05) as shown in Table 2. There was no significant difference in the levels of plasma E1, E2 and FSH between the NH group and CG group before treatment (p<0.05). Compared with the NH group, the plasma levels of E1 and E2 in CG group was significantly higher after treatment, while compared to the CG group, the level of serum FSH in NH group was significantly higher after treatment (p<0.05) as shown in Table 3. There was no significant difference in tumor size between the NH group and CG group before treatment (p>0.05). After treatment, the tumor volume of each group gradually decreased with increasing treatment time, and the tumor reduction degree of CG group was lower than that of NH group (p<0.05) as shown in Table 4. The number of leucopenia cases in the NH group was 14, and that in the CG group were 17, slightly more than that in the NH group, but not significantly higher as shown in Table 5. After 2 y of follow-up, the survival rate of NG group was significantly higher than that of CG group (p<0.05) as shown in figure 1.
Table 2: Clinical Efficacy of the NH and CG Groups
|Group||n||Complete remission||Partial remission||Disease stabilization||Total effective rate|
|Group Nh||68||9 (13.2 %)||35 (51.4 %)||2 (33.8 %)||44 (64.7 %)|
|Group Cg||68||4 (5.8 %)||12 (17.6)||39 (57.3 %)||16 (23.5 %)|
Table 3: Changes in Serum E1, E2 and FSH in NH and CG Groups
|Group||n||E1 (pg/ml)||E2 (pg/ml)||FSH (mIU/ml)|
|Before treatment||After treatment||Before treatment||After treatment||Before treatment||After treatment|
#Means comparison with the same group before treatment (p<0.05)
Table 4: Tumor Size After 1-3 Courses of Treatment in the NH and CG Groups
|Group||n||Before treatment||First course of treatment||Second course of treatment||F||P|
*Means comparison with the pretreatment (p<0.05), #means comparison with the first course (P<0.05), *means comparison with the second course (p<0.05)
Table 5: Adverse Reactions of the NH and CG Groups
|Group||n||Nausea and vomiting||Alopecia||Leukopenia||Central granulocytopenia||Peripheral neurotoxicity|
Over the years, breast cancer showed a high mortality rate. Every year, more than 200 000 women are diagnosed with breast cancer all over the world. The causes of breast cancer are complex and involve many factors. However, at present, there are very limited surgical and therapeutic interventions available for treating breast cancer. In addition, chemotherapy drugs in clinical use currently have certain toxicity and lead to many side effects. At the same time drug resistance towards chemotherapy drugs also appear in patients with adenocarcinoma[9,10]. Similar to the occurrence of other tumors, breast cancer development is a longterm and multi-step process, involving complex genetic and epigenetic changes. Although in the past few decades, many studies have explored the pathogenesis of breast cancer, at present, the molecular mechanism of the occurrence and development of breast cancer is still poorly understood. The clinical outcome of patients after surgery is poor, and the long-term prognosis is not ideal.
In this study, it was found that in the NH group there were 9 complete remission cases, 35 partial remission cases, 23 disease stable cases while, 8 cases showed disease progression. On the other hand in the CG group, only 4 complete remission cases, 12 partial remission cases, 39 disease stable cases and 13 disease progression cases were seen. The total effective rate of NH group was higher than that of CG group. Compared with the NH group, the serum E1 and E2 levels of the CG group were significantly higher, and compared with the CG group, the serum FSH levels of the NH group were significantly higher. It was reported that many hormones such as E1, E2, FSH in the female can promote the proliferation and invasion of cancer cells and the mechanism may be related to the activation of proteins, enzymes or oncogenes involved in nucleic acid synthesis induced by these hormones, so as to increase the growth of cancer cells[13,14]. Conventional macromolecular chemotherapy drugs are difficult to be absorbed. Low concentration chemotherapy drugs have little effect on cancer cells. Epirubicin, an anthracycline drug, would show a higher concentration in the tumor, which could kill the cells before these invade other organs, fundamentally reducing the possibility of recurrence and metastasis of cancer[15,16]. Some studies have confirmed that epirubicin has outstanding ability to penetrate into cancer cells. After embedding into DNA base pairs, it can closely combine with DNA resulting in the reduction of DNA replication leading to reduction in DNA-dependent polymerase activity and RNA transcription, thus inhibiting tumor growth with in, which is consistent with the results obtained in this investigation.
In this study, it was found that the number of neutropenia cases in the NH group was 14 at most, and the number of neutropenia cases in the CG group was 17, and the number of adverse reactions in the CG group was significantly higher than that in the NH group. The survival rate of the patients in the NH group was significantly higher than that in the CG group. After treatment, the tumor volume in each group decreased gradually with increasing treatment time and the tumor reduction degree of CG group was lower than that of NH group. According to reported literature, chemotherapy could inhibit tumor neovascularization and different drugs have different effects, but could induce tumor cell apoptosis. After treatment, tumor volume could be significantly reduced. The clinical outcome of single use or combined use is considerable and the incidence of cardiotoxicity of epirubicin is significantly lower than that of Adriamycin. Some studies have confirmed that the combination of epirubicin with other drugs has a significant effect in the treatment of breast cancer. Compared to conventional chemotherapy drugs, the adverse reactions are few and the prognosis is better, which is worth promoting its clinical use. To sum up, anthracycline is effective in the treatment of advanced breast cancer, the level of serum E2 is decreased and the tumor volume is reduced.
Conflict of interest
Authors reported no conflicts of interest.
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