Abstract
In Situ Single-Pass Intestinal Perfusion in Rats for Intestinal Permeability Investigation of Drugs
Department of Pharmacy, Zhejiang Provincial People’s Hospital Bijie Hospital, Bijie 551700, 1School of Pharmaceutical Sciences, Nanjing Tech University, 2Jiangsu Provincial Institute of Materia Medica, Nanjing 211816, PR China
Correspondence Address:
Hongqun Qiao, School of Pharmaceutical Sciences, Nanjing Tech University, 2Jiangsu Provincial Institute of Materia Medica, Nanjing 211816, PR China, E-mail: qiaohongqun@njtech.edu.cn
The aim of this study was to establish the in situ single-pass intestinal perfusion method in rats for intestinal permeability investigation of drug. Effective permeability coefficients (Peff) of 15 model drugs were investigated in rat jejunal. Krebs-Ringer buffer solution containing drug was perfused at flow rate of 0.2 ml/min and samples were taken from outlet up to 110 min, steady state was achieved after 30 min perfusion without samples taken. Gravimetric method was used to correct the net water flux. Drug concentrations in perfusion samples were determined using high-performance liquid chromatography-ultraviolet method and Peff were calculated. The Peff values obtained by in situ single-pass intestinal perfusion ranged from 0.0146±0.0026×10-4 cm/s of enalaprilat to 1.77±0.34×10-4 cm/s of antipyrine. Among the selected model drugs, the Peff values of high permeability drugs were higher than 0.2×10-4 cm/s, and less than 0.03×10-4 cm/s for low permeability drugs. The experimental rat Peff was highly correlated to the literature human Peff with correlation coefficient (R2) of 0.99. The observed experimental rat Peff values were highly correlated to the literature human Fabs (R2=0.93). All investigated model drugs have similar permeability classification compare with literature permeability classification. The predicted human Fabs were linearly correlated with literature human Fabs (R2=0.90). This method could be used for investigation of the intestinal absorption of new compounds, and for prediction the absorption fraction in human. Besides, investigation of the permeability classification of generic drug by in situ single-pass intestinal perfusion can be used for application of biowaiver for industry.
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